AMP™ Real-Time PCR SMART Mix is an optimized system allowing the use of an Fluorescent intercalating agent in a real-time PCR amplification assay. The use of AMP™ h-Taq DNA Polymerase allows to obtain accurate and reliable results. The AMP h-Taq is an HotStart Polymerase which enables target-specific amplification, primer-dimer or non-specific binding is thus prevented.
The SYBR® Green I fluorescent dye is incoporated into the mix and used as an intercalating reagent. High resolution melting (HRM) analysis is thus applicable for genetic analysis such as SNP genotyping, mutation discovery or CpG methylation.
DNA quantification using real-time PCR.
Real-Time quantification of DNA templates.
High Resolution Melting (HRM) analysis.
2X Real-Time PCR Smart mix (Including SYBR® Green I in the mixture) :
Stable for 6 months in the case of storage at -20°C, or
7 days at 25℃ before activity begins to reduce.