BAC-Dent 2.4

DNA based identification of bacteria associated with periodontitis

  • Range of Detection
    Simultaneous detection of 11 bacterial species associated with human
    periodontitis.

 

  • Principle
    Regions of the bacterial 16S rRNA gene are amplified in one ‘Triplex’ reaction and are subsequently detected by hybridization to immobilized, species specific capture probes. The short amplicon lengths (85 to 350 bp) ensure high sensitivity.

 

  • Fast Protocol
    optimized 45 minutes protocol including analysis (post amplification)

 

  • Simple & Robust
    non-fluorescent detection for easy read-out, minimal lab instrumentation required

 

Product Information

 

Assay Principle

Biotinylated PCR products are generated from sample material containing extracted bacterial DNA from subgingival plaques with the provided primer mix (Triplex-PCR). The labeled amplicons are hybridized to species specific capture probes immobilized on the LCD-Chip surface. Following a short wash routine, comprising high stringency, visualization of bound amplicons is mediated by an enzyme-substrate cascade. Each LCD-Chip contains eight identical micro arrays separated in small reaction chambers. Each array can be addressed individually, allowing the parallel analysis of eight samples on one chip.

 

Target Region & Sensitivity

The provided primer recognize highly conserved motifs in the 16S_rRNA gene of the bacterial genomes. The short amplicon lengths of ~80, 300 and 350 bp guarantees excellent sensitivity. Specific capture probes immobilized in duplicate permit the simultaneous detection of the 11 most important bacterial species associated with human periodontitis. Following PCR amplification the complete procedure takes 45 minutes. Data analysis can be achieved using the SlideReader software or by simple examination with the naked eye.

 

schema

Fig. 1

tableau

Fig. 2



Array Pattern

Fig.1:
fig1
Parallel analysis of eight samples on one LCD-Chip. The array field spacing is compatible with standard multi pipettes.
Fig.2 :

fig2
Analysis of two DNA preparations from human subgingival plaques
A) A. actinomycetemcomitans, T. forsythia, C. rectus, C. gingivalis, Fusabacterium sp.Prevotella intermedia, Tannerella forsythensis, Fusobacterium sp. and control (E.coli).
B) P. gingivalis, T. forsythia, C. rectus, P. micros, Fusobacterium sp. and control (E.coli).

 

Software

slidereader

SlideReader Vers. 9

• Fully automated image analysis
• comprehensive data reports in PDF format
• fast & intuitive navigation
• multiple user accounts

 

Instrumentation

instrumentation

CHIP-Scanner PF3650u

• Transmission light scanner for LCD-Arrays,
• 10 µm resolution
• 16 bit grey scale TIFF images

CHIP Spin FVL2400

• Bench Top mini centrifuge
• 2400 rpm, constant
• Adaptor for LCD-Arrays
• incl. 3 rotors

Order Information Cat. No.
BAC-Dent 2.4 LCD-Array Kit, 96 Tests B-400-12
BAC-Dent 2.4 LCD-Array Kit, 32 Tests B-400-04
CHIP-Scanner PF2700 HS-500-01
CHIP-Spin FVL2400 HS-300-01
SlideReader Software Vers. 7.00.01 HS-200-01

BAC DENT