DNA based identification of bacteria associated with periodontitis
Biotinylated PCR products are generated from sample material containing extracted bacterial DNA from subgingival plaques with the provided primer mix (Triplex-PCR). The labeled amplicons are hybridized to species specific capture probes immobilized on the LCD-Chip surface. Following a short wash routine, comprising high stringency, visualization of bound amplicons is mediated by an enzyme-substrate cascade. Each LCD-Chip contains eight identical micro arrays separated in small reaction chambers. Each array can be addressed individually, allowing the parallel analysis of eight samples on one chip.
The provided primer recognize highly conserved motifs in the 16S_rRNA gene of the bacterial genomes. The short amplicon lengths of ~80, 300 and 350 bp guarantees excellent sensitivity. Specific capture probes immobilized in duplicate permit the simultaneous detection of the 11 most important bacterial species associated with human periodontitis. Following PCR amplification the complete procedure takes 45 minutes. Data analysis can be achieved using the SlideReader software or by simple examination with the naked eye.
Parallel analysis of eight samples on one LCD-Chip. The array field spacing is compatible with standard multi pipettes.
Analysis of two DNA preparations from human subgingival plaques
A) A. actinomycetemcomitans, T. forsythia, C. rectus, C. gingivalis, Fusabacterium sp.Prevotella intermedia, Tannerella forsythensis, Fusobacterium sp. and control (E.coli).
B) P. gingivalis, T. forsythia, C. rectus, P. micros, Fusobacterium sp. and control (E.coli).
SlideReader Vers. 9
• Fully automated image analysis
• comprehensive data reports in PDF format
• fast & intuitive navigation
• multiple user accounts
• Transmission light scanner for LCD-Arrays,
• 10 µm resolution
• 16 bit grey scale TIFF images
CHIP Spin FVL2400
• Bench Top mini centrifuge
• 2400 rpm, constant
• Adaptor for LCD-Arrays
• incl. 3 rotors
Order Information Cat. No.
BAC-Dent 2.4 LCD-Array Kit, 96 Tests B-400-12
BAC-Dent 2.4 LCD-Array Kit, 32 Tests B-400-04
CHIP-Scanner PF2700 HS-500-01
CHIP-Spin FVL2400 HS-300-01
SlideReader Software Vers. 7.00.01 HS-200-01