OneStep qRT-PCR in a single tube using probe
High specific detection using RTase and h-Taq DNA polymerase (HotStart)
Applicable to detect of various RNA sample
Ready to use Smart mix type
DAQ™ 2X OneStep Multiplex qRT-PCR Kit for Probe is designed to perform real-time RT-PCR in a single tube at OneStep for cost- and time-saving. Included thermostable DAQ™ RTase and AMP™ h-Taq DNA Polymerase mixture enables ultra specific and accurate detection and amplification of target RNA by its optimal activation temperature (50°C) and HotStart activation characteristics to help inhibition of non-specific amplification. DAQ™ 2X OneStep Multiplex qRT-PCR Kit for Probe is the best optimized to a system which is using sequence specific designed probe from various RNA samples. The product is provided as a ready-to-use Smart mix type which is including enzyme mixture, buffer, and dNTP as a 2X concentration.
RNase H: M-MLV RTase (RTase H-).
Easy sample optimization.
Temperature of synthesis cDNA: 42 – 55℃.
Synthesis length: < 1 kb.
Synthesis primer: Gene specific primer.
Source: Thermus aquaticus (AMP™ h-Taq).
Amplification size: < 1 kb.
Real-Time Quantification of RNA target.
Application using qPCR.
2X OneStep Multiplex qRT-PCR enzyme mixture
(RTase +AMP™ h-Taq DNA Polymerase).
5X OneStep Multiplex qRT-PCR Reaction Buffer
(with dNTP Mix).
Stable for 1 year in the case of storage at -20, or
7 days at 25℃ before activity begins to reduce.