Fast and easy OneStep RT-PCR reaction in a single tube
Dual advantage of DAQ™ RTase and DAQ™ h-Taq DNA Polymerase (HotStart)
Synthesis cDNA at 50℃
DAQ™ OneStep RT-PCR Kit is designed to synthesise cDNA from RNA and its amplification in a single tube at OneStep to save time and cost. DAQ™ RTase and AMP™ h-Taq DNA Polymerase present in an optimized mixture helps to get PCR product with greater efficiency and high specificity. The optimal activation temperate (50°C) of RTase and HotStart activity of h-Taq helps inhibit the non-specific amplification of target DNA. The optional 5X Band Doctor™ helps amplification of difficult PCR caused by GC-rich or complex template structure. DAQ™ OneStep RT-PCR Series is provided in three types as for easy-use: standard kit (enzyme mixture, buffer, 5X Band Doctor™, dNTP mixture, RNase-free water), Smart mix (master mix in 1.5ml tube) and Pre-Mix (master mix in 0.2ml 8-strip tube).
RTase: M-MLV RTase (RTase H-).
Temperature of synthesis cDNA: 42 – 55℃.
Synthesis length: < 1 kb.
Synthesis primer: Gene specific primer.
Source: Thermus aquaticus (AMP™ h-Taq).
Amplification size: < 1 kb.
DAQ™ OneStep RT-PCR enzyme mixture.
(DAQ™ RTase + AMP™ h-Taq DNA Polymerase).
5X OneStep RT-PCR Reaction Buffer.
5X Band Doctor™.
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP).
Stable for 1 year in the case of storage at -20, or
7 days at 25℃ before activity begins to reduce.