Dengue 1.5

DNA based identification of all 4 Dengue Virus Types

  • Sensitivity
    Based on the amplification of a ~ 150 bp fragment from the “non coding”
    3’ end of the Dengue Virus RNA, the assay shows excellent sensitivity

 

  • Specificity
    2 universal Dengue Virus capture probes are included to detect all known varieties
    3 Type specific capture probes allow to discriminate between the 4 Dengue Virus Types
    3 additional capture probes for each Type provide strain specific information

 

  • Fast Protocol
    optimized 45 minutes protocol including analysis (post amplification)

 

  • Simple & Robust
    Non-fluorescent detection for easy read-out, minimal lab instrumentation required

 

Product Information

 

Assay Principle

Biotinylated PCR* products are generated from sample material containing extracted viral RNA by a „One-Tube RT-PCR“ protocol. The labeled amplicons are hybridized to Dengue Virus specific capture probes immobilized on the LCD-Chip surface. Following a short wash routine, comprising high stringency, visualization of bound amplicons is mediated by an enzyme-substrate cascade. Each LCD-Chip contains eight identical micro arrays separated in small reaction chambers. Each array can be addressed individually, allowing the parallel analysis of eight samples on one chip

 

Target Region & Sensitivity

The provided primer recognize highly conserved motifs in the “noncoding” 3’ end of the viral RNA. The short amplicon length of ~150bp guarantees excellent sensitivity. 17 specific capture probes ensure the detection of all known varieties of Types 1-4. Apart from universal probes for Dengue viruses, and general Type specific probes, each Dengue Virus Type is represented by three capture probes complementary to highly variable regions which enable to gain strain specific information.

array

Array Pattern

Fig.1:

fig1
Parallel analysis of eight samples on one LCD-Chip. The

Fig.2 :

fig2Detection of two different Dengue Virus isolates**.
A) Dengue Virus Type 1
B) Dengue Virus Type 4
** kindly provided by Dr. C. Drosten, BNI Hamburg, Germany.

* The Polymerase Chain Reaction (PCR) process is covered by U.S. patents owned by Hoffmann-La Roche, Inc. and patents owned by F. Hoffmann-La Roche Ltd. No license under these patents to use the PCR process is conveyed expressly or by implication to the purchaser by the purchase of this product.

 

Software

software

SlideReader Vers. 7.01

• Fully automated image analysis
• comprehensive data reports in HTML format
• fast & intuitive navigation
• multiple user accounts

 

Instrumentation

instrumentation

CHIP-Scanner PF2700

• Transmission light scanner for LCD-Arrays,
• 10 µm resolution
• 16 bit grey scale TIFF images

CHIP Spin FVL2400

• Bench Top mini centrifuge
• 2400 rpm, constant
• Adaptor for LCD-Arrays
• incl. 3 rotors

Order Information Cat. No.
DENGUE 1.4 LCD-Array Kit, 48 Tests V-200-06
CHIP-Scanner PF2700 HS-500-01
CHIP-Spin FVL2400 HS-300-01
SlideReader Software Vers. 7.00.01 HS-200-01

dengue