DiaPlexC™ ApoE Genotyping Kit (Conventional)

The Apolipoprotein E (ApoE) Genotyping Kit is designed to screen for Alzheimer’s disease using a real-time or conventional allele-specific Multiplex PCR with human genomic DNA.

Pathogen Information

Apolipoprotein E (ApoE) consists of three isoforms: E2, E3 and E4. These three isoforms make six genotypes, (homozygous genotypes: E2/E2, E3/E3 E4/E4 and heterozygous genotypes: E2/E3, E2/E4, E3/E4). Isoform E2 is usually known as a protective isoform whereas isoform E4 is generally responsible for diseases. The predominant abnormalities are due to a single nucleotide polymorphism (SNP) of codon 112 (Cys/Arg) and codon 158 (Arg/Cys). The polymorphism has functional effects on the lipoprotein metabolism. ApoE is the primary ligand for two receptors, LDL receptors found in the liver and other tissues and ApoE specific receptors found in the liver. The co-ordinate interaction of these lipoprotein complexes with their receptors forms the basis for the metabolic regulation of cholesterol. ApoE has been found to be associated with elevated cholesterol levels or lipid derangements that lead to various clinical problems like coronary heart diseases, strokes, periphery artery diseases, and diabetes mellitus. In addition to the genotype-phenotype associations with the cardiovascular disease, the alleles and isoforms of ApoE have been related to dementias, most commonly Alzheimer’s disease. Determining the genotype of ApoE is thus considered to be an important test for patients with the disease, as well as a precautionary test for healthy people.

Detection Technology

Conventional PCR

Specimens

Blood, Buccal epithelial cell, Hair (root)

Target Genes

ApoE genotype (E2/E2, E3/E3, E2/E4, E3/E3, E3/E4, E4/E4)

Features

HotStart PCR System: Ultra highly-specific and sensitive results.
Multiplex PCR: Multiple targets in a single reaction.
Reliable System: Internal control.
Easy-to-use Master Mix: Just add the template and primer.
CE Certification.

Contents

2X Multiplex PCR Smart mix
Primer mixture
Standard marker
Control templates (E2/E4)
Nuclease free water

References

1.Lepara O, Valjevac A, Alajbegovic A, Zaciragic A, Nakas-Icindic E (August 2009). “Decreased serum lipids in patients with probable Alzheimer’s disease”. Bosn J Basic Med Sci 9 (3):215-20

2.Golomb BA, Evans MA (2008). “Statin adverse effects : a review of the literature and evidence for a mitochondrial mechanism”. Am J CardiovascDrugs 8 (6):373-418

3.Hoek KS, Schlegel NC, Eichhoff OM, et al. (2008). “Novel MITF targets identified using atwo-step DNA microarray strategy”. Pigment Cell Melanoma Res. 21 (6):665-76

4.Singh PP, Singh M, Mastana SS (2006). “ApoE distribution in world populations with new data from India and the UK.”. Ann.Hum. Biol. 33 (3)279-308