DiaPlexC™ Bacteria Detection Kit (Conventional)

The DiaPlexC™ Bacteria Species Detection Kit is designed to detect Bacteria 85 species (Enterobacter sp., Steptococcus sp., Bacilus sp., Camplilobacteria sp., Klebsiella sp., Mycobacterium sp., etc) from CELL THERAPY products using a multiplex PCR technology which can detect multiple specific target genes in a single PCR.

Pathogen Information

Bacteria are widespread cell culture contaminants. These are usually introduced through poor aseptic technique and are often first recognized in culture medium. Sources of contamination include the cells themselves, the media or serum, and airborne contamination. Even when excellent aseptic technique is in place, it is essential to monitor and test for contamination. In the absence of antibiotics, they will grow rapidly. However if antibiotics are routinely used, low level infections may develop that may be more difficult to observe.

Detection Technology

Conventional PCR

Specimens

Culture media, Suspension media
In case of biomedical products, raw materials or finished products.

Target Organisms

Bacteria of 85 species
(Enterobacter sp., Steptococcus sp., Bacilus sp., Cam
plilobacteria sp., Klebsiella sp., Mycobacterium sp. etc)

Features

HotStart PCR System: Ultra highly-specific and sensitive results.
DnaFree™ System: No host genomic DNA contamination.
UDG System: No carryover contamination.
Multiplex PCR: Multiple targets in a single reaction.
Reliable System: Automatic PCR control.
Easy-to-use Master Mix: Just add the template and primer.

References

1. Park SH, Kim HJ, Kim JH, Kim TW, Kim HY. Simultaneous
detection and identification of Bacillus cereus group bacteria
using multiplex PCR. J Microbiol Biotechnol. 2007 Jul;17(7):1
177-82.

2. Aksu G, Ruhi MZ, Akan H, Bengisun S, Ustün C, Arslan O,
Ozenci H. Aerobic bacteria and fungal infections in periphe
ral blood stem cell transplants. Bone Marrow Transplant.
2001 Jan;27(2):201-5.

3. Warwick S, Wilks M, Hennessy E, Powell-Tuck J, Small M,
Sharp J, Millar MR. Use of quantitative 16S ribosomal DNA
detection for diagnosis of central vascular catheter-assoc
iated bacteria infection. J Clin Microbiol. 2004 Apr;42 (4):
1402-8.

4. Nele Wellinghausen, Anna-Julia Kochem, Claudia Disqué,
Helge Mühl, Susanne Gebert, Juliane Winter, Jens Matten,
and Samir G. Sakka. Diagnosis of Bacteremia in Whole-
Blood Samples by Use of a Commercial Universal 16S
rRNA Gene-Based PCR and Sequence Analysis. J Clin
Microbiol. 2009 September; 47(9): 2759–2765.

Contents

2X Multiplex PCR Smart mix
Primer mixture
Standard marker
Control templates
Nuclease-free water