DiaPlexC™ CRE Detection Kit

The DiaPlexC™ CRE Detection Kit is designed to detect 4 types of CRE genes, VIM, IMP, NDM and KPC, which code metallo-β-lactamase-1, using the Multiplex PCR technology which can detect multiple specific target genes in a single PCR.

Pathogen Information

Carbapenem-Resistant Enterobacteriaceae (CRE) is a type of gram-negative bacteria that is resistant to the antibiotics related to the Carbapenem. Unguided or prolong use of antibiotics use of antibiotics lead some bacteria to become insensitive due to the generation of Metallo-β-lactamase. The carbapenemase gene is known to include the KPC, NDM, VIM, IMP, SIM, GES, SPM and OXA gene families. Its severity came to the forth as the first reported infected patient from NDM-1 (New Delhi Metallo-β-lactamase-1) and quickly spread worldwide. In particular, the NDM-1 and KPC-2 gene exists in a plasmid of some strains that can be propagated easily into heterogeneity by conjunction.

The European Union and the World Health Organization recognize the emergence of antibiotic-resistant strains as a serious situation, and try to prevent its spread by strict management through ongoing monitoring and research development. CRE is now a global challenge and has been associated with high rates of morbidity and mortality. Therefore, the accurate and fast detection of the carbapenem family of antibiotic-resistant genes is very important.

Detection Technology

Conventional PCR

Specimens

Urine, Bile acid, Sputum, Blood, Gastric juice or Stool.

Target Organisms

VIM (Verona ingetron-encoded Metallo-β-lactamase-1)
IMP (Imipenem-resistant P.aeruginosa)
NDM (New Delli Metallo β-lactamease)
KPC (Klebsiella pneumonia carbapenemase)
Analytical Sensitivity
102 copies

Features

HotStart PCR System: Ultra highly-specific and sensitive results.
DnaFree™ System: No host genomic DNA contamination.
UDG System: No carryover contamination.
Multiplex PCR: Multiple targets in a single reaction.
Reliable System: Automatic PCR control.
Easy-to-use Master Mix: Just add the template and primer.
CE Certification.

Contents

2X Multiplex PCR Smart Mix
Primer Mixture
Standard Marker
Control Templates
Nuclease-free Water

References

1.Schwaber MJ, Carmeli Y. Carbapenem-resistant Enterobacteriaceae:a potential threat. JAMA 2008;300:2911–3.

2.CDC, Healthcare Infection Control Practices Advisory Committee. Management of multidrug-resistant organisms in healthcare settings, 2006. Atlanta, GA: US Department of Health and Human Services, CDC,Healthcare Infection Control Practices Advisory Committee; 2007

3.Srinivasan A, Patel JB. Klebsiella pneumoniae carbapenemase-producing organisms: an ounce of prevention really is worth apound of cure. Infect Control Hosp Epidemiol 2008;29:1107–9.

4.Patel G, Huprikar S, Factor SH, Jenkins SG, Calfee DP. Outcomes of carbapenem-resistant Klebsiella pneumoniae infection and the impact of antimicrobial and adjunctive therapies. Infect ControlHospEpidemiol 2008;29:1099–106