The DiaPlexC™ CRE Detection Kit is designed to detect 4 types of CRE genes, VIM, IMP, NDM and KPC, which code metallo-β-lactamase-1, using the Multiplex PCR technology which can detect multiple specific target genes in a single PCR.
Carbapenem-Resistant Enterobacteriaceae (CRE) is a type of gram-negative bacteria that is resistant to the antibiotics related to the Carbapenem. Unguided or prolong use of antibiotics use of antibiotics lead some bacteria to become insensitive due to the generation of Metallo-β-lactamase. The carbapenemase gene is known to include the KPC, NDM, VIM, IMP, SIM, GES, SPM and OXA gene families. Its severity came to the forth as the first reported infected patient from NDM-1 (New Delhi Metallo-β-lactamase-1) and quickly spread worldwide. In particular, the NDM-1 and KPC-2 gene exists in a plasmid of some strains that can be propagated easily into heterogeneity by conjunction.
The European Union and the World Health Organization recognize the emergence of antibiotic-resistant strains as a serious situation, and try to prevent its spread by strict management through ongoing monitoring and research development. CRE is now a global challenge and has been associated with high rates of morbidity and mortality. Therefore, the accurate and fast detection of the carbapenem family of antibiotic-resistant genes is very important.
Urine, Bile acid, Sputum, Blood, Gastric juice or Stool.
VIM (Verona ingetron-encoded Metallo-β-lactamase-1)
IMP (Imipenem-resistant P.aeruginosa)
NDM (New Delli Metallo β-lactamease)
KPC (Klebsiella pneumonia carbapenemase)
HotStart PCR System: Ultra highly-specific and sensitive results.
DnaFree™ System: No host genomic DNA contamination.
UDG System: No carryover contamination.
Multiplex PCR: Multiple targets in a single reaction.
Reliable System: Automatic PCR control.
Easy-to-use Master Mix: Just add the template and primer.
2X Multiplex PCR Smart Mix
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