DiaPlexC™ Fungi Detection Kit (Conventional)

The DiaPlexC™ Fungi Detection Kit is designed to detect 16 species of Fungi present in cell cultures using the Multiplex PCR technology which can detect multiple specific target genes in a single PCR.

Pathogen Information

Fungi are one of the major ubiquitously found contaminants in the laboratory. It can contaminate any kind of biological related materials and spoil them. In the laboratory, cell cultures, culture media, broth, serum and plasma are all highly susceptible to the fungal contamination unless strict aseptic measures are applied. Even when excellent aseptic technique is applied, it is essential to monitor and test to confirm results are free from contamination. In the absence of antibiotics they will grow rapidly; however, if antibiotics are routinely used low levels of fungal infections may develop that might be more difficult to observe.

Detection Technology

Conventional PCR

Specimens

Culture media, Suspension media, Raw material and Final material of Biopharmaceuticals.

Target Organisms

Fungi of 16 species

Analytical Sensitivity

10-103 copies

Features

HotStart PCR System: Ultra highly-specific and sensitive results.
DnaFree™ System: No host genomic DNA contamination.
UDG System: No carryover contamination.
Multiplex PCR: Multiplex targets in a single reaction.
Reliable System: Automatic PCR control.
Easy-to-use Master Mix: Just add the template and primer.

Contents

2X Multiplex PCR Smart mix
Primer mixture
Standard marker
Control templates
Nuclease-free Water

References

1. Judith Behnsen, Andrea Hartmann, Jeannette Schmaler, Alexander Gehrke, Axe A. Brakhage, and Peter F. Zipfe. (2008) The Opportunistic Human Pathogenic Fungus Aspergillus fumigatus Evades the Host Complement System. Infect. Immun. 2008. vol. 76 no. 2 820-827

2. Mahmoud A. Ghannoum, Richard J. Jurevic, Pranab K. Mukherjee, Fan Cui, Masoumeh Sikaroodi, Ammar Naqvi, Patrick M. Gillevet. Characterization of the Oral Fungal Microbiome (Mycobiome) in Healthy Individuals. 2010. Vol 6

3. The NIH Office of Technology Transfer (OTT). Adhesion Molecules (Carbohydrate Receptors) For Pathogenic Bacteria And Fungi.

4. Luo G, Mitchell TG. Rapid identification of pathogenic fungi directly from cultures by using multiplex PCR. J Clin Microbiol. 2002 Aug;40(8):2860-5.

5. Martin C, Roberts D, van Der Weide M, Rossau R, Jannes G, Smith T, Maher M. Development of a PCR-based line probe assay for identification of fungal pathogens. J Clin Microbiol. 2000 Oct;38(10):3735-42.