DiaPlexC™ Influenza Virus A Subtype Detection Kit

The DiaPlexC™ Influenza Virus A Subtype Detection Kit is designed to detect 6 types of Influenza Virus A Subtype pathogens using multiplex RT-PCR technology which can detect multiple specific target genes in a single PCR.

Pathogen Information
Influenza virus A (H1N1) is a subtype of Influenza virus A and the most common cause of influenza (flu) in humans. Some strains of H1N1 are endemic in humans and cause a small fraction of all influenza-like illness and a large fraction of all seasonal influenza. H3N2 is currently endemic in both human and pig populations. It evolved from H2N2 through an antigenic shift and caused the Hong Kong Flu pandemic of 1968 and 1969 that killed almost 750,000 people. The dominant strain of annual flu in January 2006 was H3N2. Since November 2003, nearly 400 cases of human infection with the highly pathogenic Avian Influenza A (H5N1) viruses have been reported by more than a dozen countries in Asia, Africa, the Pacific, Europe and the Near East. Highly pathogenic Avian Influenza A (H5N1) virus infections occur in both poultry and humans. Furthermore, although H7N2, H7N3, H7N7 and H9N2 are classified as relatively low pathogenic infections, they have been reported in humans in the past few years and they can potentially develop into pandemic infections.

Detection Technology
Conventional PCR

 

Specimens
Nasopharyngeal swab, Nasopharyngeal aspirate.
Bronchoalveolar lavage (BAL).
Target Organisms
H1N1-Seasonal H5N1
H1N1-Pandemic(2009) H7 common
H3N2 H9N2
Analytical Sensitivity
10-102 copies.

 

Features
HotStart PCR System: Ultra highly-specific and sensitive results.
Multiplex RT-PCR: Multiple targets in a single reaction.
Reliable System: Internal control.
Easy-to-use Master Mix: Just add the template and primer.
CE Certification.
Contents
2X Multiplex OneStep RT-PCR Smart Mix
Primer Mixture
Standard Marker
Nuclease-free Water

 

References
1.Lin YP, Shaw M, Gregory V, Cameron K, Lim W, Klimov A, et al. Avian-to-human transmission of H9N2 subtype influenza A viruses: relationship between H9N2 and H5N1 human isolates. Proc Natl Acad Sci USA. 2000; 97:9654-58

2.CDC, Interim guidance on specimen collection, processing, and testing for patients with suspected novel influenza A(H1N1) virus infection, May 13, 2009