DiaPlexC™ Influenza Virus A/B Detection Kit

The DiaPlexC™ Virus A/B Detection Kit is designed to detect influenza virus A and B using multiplex RT-PCR technology which can detect multiple specific target genes in a single PCR.

Pathogen Information
Influenza is a family of RNA enveloped viruses that affect mammals and birds. Among three types of influenza viruses A, B &C, Influenza A is the most important in human infection. Influenza is a huge public health concern that occurs in seasonal epidemics and has caused pandemic level infections.

There are three types of influenza viruses; A, B and C, in which specificity is conferred by internal nucleoprotein and matrix protein antigens. Influenza A and B are the major causative agent of human acute respiratory disease worldwide, while Influenza virus C occurs much less frequently than A and B, That is why only influenza A and B are included in seasonal influenza vaccines.

 

Detection Technology
Conventional PCR

 

Specimens
Nasopharyngeal swab, Nasopharyngeal aspirate
Bronchoalveolar lavage (BAL)

 

Target Organisms
Influenza virus A
Influenza virus B

 

Analytical Sensitivity
10-102 copies.
Features
HotStart PCR System: Ultra highly-specific and sensitive results.
Multiplex RT-PCR: Multiple targets in a single reaction.
Reliable System: Automatic PCR control.
Easy-to-use Master Mix: Just add the template and primer.
CE Certification.
Contents
OneStep RT-PCR Enzyme Mix.
2X OneStep RT-PCR Buffer.
Primer Mixture.
Standard Marker.
Control Template.
RNase-free Water.
References
1.Fouchier RAV, Munster A, Wallenstein TM, Bestebroer S, Herfst D, Smith GF, et al. J Virol. 2002;79:2814-2822

2.Lamb RA, Krug R. Field Virology, third ed. Lippincott-Raven Philadelphia. 1996;1353-1395.

3.Ha Y, Stevens, DJ, Wiley DC. The EMBO Journal. 2002;21;865-875

4.Russell RJ, Gamblin SJ, Haire LF, Stevens DJ, Xiao B, Ha Y, et al. Virology. 2004;287-296