DiaPlexC™ M.Avium/M.Intracellulare Detection Kit

The DiaPlexC™ M.Avium/M.Intracellulare Detection Kit is designed to detect Mycobacterium Avium and mycobacterium Intracellulare of Non-Tuberculosis Mycobacterium using the Multiplex PCR technology which can detect multiple specific target genes in a single PCR.

Pathogen Information

Mycobacterium avium and Mycobacterium intracellulare complex (MIC) is the atypical mycobacterium most commonly associated with human disease. MIC is primarily a pulmonary pathogen that affects individuals who are immune compromised (eg, from AIDS, hairy cell leukemia, immunosuppressive chemotherapy). In this clinical setting, MIC has been associated with osteomyelitis; tenosynovitis; synovitis. MIC comprises two genetically distinct but difficult to discriminate species. M.avium predominates (87-98%) in AIDS patients with no anti-retroviral therapy and M.intracellulare is more frequent among non-AIDS patients.

Detection Technology

Conventional PCR

Specimens

Nasopharyngeal swab, Nasopharyngeal aspirate
Bronchoalveolar lavage (BAL), Nasal aspirates or Sputum
Target Organisms
Mycobacterium Avium
Mycobacterium Intracellulare
Analytical Sensitivity
102 Copies

Features

HotStart PCR System: Ultra highly-specific and sensitive results.
DnaFree™ System: No host genomic DNA contamination.
UDG System: No carryover contamination.
Multiplex PCR: Multiple targets in a single reaction.
Reliable System: Automatic PCR control.
Easy-to-use Master Mix: Just add the template and primer.
CE Certification.

Contents

2X Multiplex PCR Smart Mix
Primer Mixture
Standard Marker
Control Templates
Nuclease-free Water

References

1. The NTM Handbook: A Guide for Patients with Nontuberculous Mycobacterial Infections Including MAC

2. Horsburgh CR Jr. Epidemiology of Mycobacterium avium complex. In: Korvick JA, Benson CA, eds. Mycobacterium avium Complex Infection: Progress in Research and Treatment. New York, NY: Marcel Dekker; 1996:1-22.

3. Tortoli E. Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. Clin Microbiol Rev. 2003;16:319-354.

4. Griffith, David E.; Aksamit, Timothy; & A. Brown-Elliott, Barbara et al. (2007). American Thoracic Society Guidelines: Diagnosis, Treatment and Prevention of Nontuberculous Mycobacterial Diseases. Am. J. Respiratory and Critical Care Medicine, Vol. 175, pp. 367-417.

5. Grange, J. M. (2007). « Environmental mycobacteria ». In Greenwood, David; Slack, Richard; Peitherer, John; & Barer, Mike (Eds.), Medical Microbiology (17th ed.), pp. 221-227. Elsevier. ISBN 978-0-443-10209-7. Evans AJ, Crisp AJ, H ubbard RB, Colville A, Evans SA, Johnston IDA. Pulmonary Mycobacterium kansasii infection: comparison of radiological appearances with pulmonary tuberculosis. Thorax. 1996;51:1243-1247