DiaPlexC™ Malaria Detection Kit

The DiaPlexC™ Malaria Detection Kit is designed to detect 4 species of the disease; Plasmodium vivax, Plasmodium falciparum, Plasmodium ovale, and Plasmodium malariae using the Multiplex PCR technology which can detect multiple specific target genes in a single PCR.

Pathogen Information

Malaria is an infectious disease caused by four types of Plasmodium species, namely P. falciparum, P. vivax, P. malariae and P. ovale. These parasites are only transmitted via the bites of infected female Anopheles mosquitoes. Malaria is endemic in almost 106 countries and global death due to the malaria infection is estimated at 1 million individuals per year. P. falciparum is a major cause of severe malaria and approximately 10-20% of the patients with falciparum malaria require urgent detection and intensive medical care. P. vivax is the second most harmful parasite of human malaria that cause more than 390 million clinical cases per year and is a chief risk factor for severe anemia among young children in most vivax-endemic areas. In general, the distribution of P. malariae coincides with that of P. falciparum in malaria-endemic areas in Africa. Although going undiagnosed in most cases of asymptomatic subclinical conditions, P. ovale is a cause of morbidity in many areas of tropical Africa.

Detection Technology

Conventional PCR

Specimens

Blood

Target Organisms

Plasmodium vivax
Plasmodium falciparum
Plasmodium ovale
Plasmodium malariae

Analytical Sensitivity

10-102 copies

Features

HotStart PCR System: Ultra high specific and sensitive results.
DnaFree™ System: No host genomic DNA contamination.
UDG System: No carryover contamination.
Multiplex PCR: Multiple targets in a single reaction.
Reliable System: Automatic PCR control.
Easy-to-use Master Mix: Just add the template and primer.
CE Certification.

Contents

2X Multiplex PCR Smart Mix
Primer Mixture
Standard Marker
Control Templates
Nuclease-Free Water

References

1.Sutherland CJ, Tanomsing N, Nolder D, et al. (May 2010). « Two nonrecombining sympatric forms of the human malaria parasite Plasmodium ovale occur globally ». J. Infect. Dis. 201 (10): 1544–50.
2.Kilama W,Ntoumi F (October 2009). « Malaria: a research agenda for the eradication era » Lancet 374 (9700):1480-2.
3.Dondorp AM, Day NP (July 2007). « The treatment of severe malaria ». Trans. R. Soc. Trop. Med. Hyg. 101 (7): 633–4.
4. »Global Malaria Mortality Between 1980 and 2010: A Systematic Analysis ». journalistsresource.org