DiaPlexC™ MTB/M.Bovis Detection Kit

DiaPlexC™ MTB/M.bovis Detection Kit is designed to detect mycobacterium tuberculosis and mycobacterium bovis using multiplex PCR technology which can detect multiple specific target genes in a single reaction PCR.

Pathogen Information

Mycobacterium tuberculosis (TB) is one of the most ubiquitously found infectious agents around the world, especially in developing countries causing pathogenesis of 8 million and the death of 2 million people each year. There is however a prevention method, the BCG(Bacillus Calmette-Guerin) TB vaccination, but it’s disadvantage is that it can not protect after 5 years from immunization, and there is also a possibility of causing diagnostic error for actual TB-infected patients by showing a false-positive result in the tuberculin test.

 

Detection Technology

Conventional PCR

 

Specimens

Nasopharyngeal swab, Nasopharyngeal aspirate
Bronchoalveolar lavage (BAL), Nasal aspirates or Sputum

 

Target Organisms

Mycobacterium Tuberculosis (MTB)
Mycobacterium bovis (M.bovis)

 

Analytical Sensitivity

102 Copies

Features

HotStart PCR System: Ultra highly-specific and sensitive results.
DnaFree™ System: No host genomic DNA contamination.
UDG System: No carryover contamination.
Multiplex PCR: Multiple targets in a single reaction.
Reliable System: Automatic PCR control.
Easy-to-use Master Mix: Just add the template and primer.
CE Certification.

Contents

2X Multiplex PCR Smart Mix
Primer Mixture
Standard Marker
Control Templates
Nuclease-free Water

References

1.”Mycobacterium tuberculosis” Sanger institute. 2007-03-29http://www.sanger.ac.uk/Projects/M_tuberculosis/. Retrieved 2008-11-16

2.Frothingham R, Meeker-Meeker-O’Connell WA. (1998). « Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats. ». Microbiology 144 (Pt 5): 1189–96.

3.Teo, SS; Shingadia, DV (2006 Jun). « Does BCG have a role in tuberculosis control and prevention in the United King dom? ». Archives of Disease in Childhood 91 (6): 529–31.