DiaPlexC™ Mycoplasma Detection Kit (Conventional)

The DiaPlexC™ Mycoplasma Detection Kit is designed to detect 65 types of the Mycoplasma species (54 Mycoplasma sp., 3 Acholeplasma, 6 Spiroplasma sp., 2 Ureaplasma., etc) in cell culture contaminants using the Multiplex PCR technology which can detect multiple specific target genes in a single PCR.

Pathogen Information

Mycoplasma infections have been relatively common in cell culture: (5-35% of all cell cultures are infected and much higher in some countries that do not practice systematic detection and elimination). Biomedical product Mycoplasma infections which are typically difficult to detect during routine cell culture work can cause physiological and morphological distortions that affect experimental results. Furthermore, a Mycoplasma infection in one cell culture has the potential to spread to yet uninfected laboratory cell cultures.

Detection Technology

Conventional PCR

Specimens

Culture media, Suspension media, Raw material and Final material of Biopharmaceuticals

Target Organisms

Mycoplasma of 65 species:
(54 Mycoplasma sp., 3 Acholeplasma sp.
6 Spiroplasma sp., 2 Ureaplasma sp., etc)

Analytical Sensitivity

10-102 copies

Features

HotStart PCR System: Ultra highly-specific and sensitive results.
DnaFree™ System: No host genomic DNA contamination.
UDG System: No carryover contamination.
Multiplex PCR: Multiple targets in a single reaction.
Reliable System: Automatic PCR control.
Easy-to-use Master Mix: Just adding template and primer.

Contents

2X Multiplex PCR Smart mix.
Primer mixture.
Standard marker.
Control templates.
Nuclease-free Water.

References

1.McGarrity G.J., Steiner, T. and Vanaman, V.Detection of Mycoplasmal Infection of Cell Cultures by DNA Fluorochro me Staining. In Methods in Mycoplasmology, Vol/ 2, J.G. Tylly and S.Razin, eds. (Academic Press, New York, 1983) pp. 183-190.

2.McGarrity G.J., Vanaman, V. and Sarama, J. Cytogenetic Effects of Mycoplasmal Infection of Cell Cultures: A Review. In Vitro 20 (1):1-18. (1984)

3.Doyle A, Gri ffi ths JB., 1998. The cell: selection and standardization. In:Cell and tissue culture: laboratory procedures in biotechnology. Doyle, A and Griffiths JB (Eds), Wiley and Sons, Ltd pp.35-52

4.Asarnow, D. et al. Validation and international regulatory experience for a mycoplasma touchdown PCR assay. Biol ogicals 38:224-231 (2010)