DiaPlexC™ RV13 Detection Kit

The DiaPlexC™ RV13 Detection Kit is designed to detect 13 major respiratory viruses using the Multiplex RT-PCR technology which can detect multiple specific target genes in a single PCR.

Pathogen Information

Viral infections are among the leading causes of respiratory disease in children. Most of these infections are caused by respiratory syncytial virus (RSV), influenza virus A or B (infA or InfB), parainfluenza virus (PIV), rhinovirus (RV), or adenovirus (AdV). Several recently discovered viruses, such as human metapneumovirus (hMPV), human bocavirus (hBoV), Enterovirus (EV) and the human coronaviruses (hCoVs) 229E and OC43, have been identified as potential respiratory pathogens.

Detection Technology

Conventional RT-PCR

Specimens

Nasopharyngeal swab, Nasopharyngeal aspirate
Bronchoalveolar lavage (BAL)

Target Organisms

Group 1
Human parainfluenza virus I (hPIV-I)
Human parainfluenza virus II (hPIV-II)
Human parainfluenza virus III(hPIV-III)
Human coronavirus OC43 (hCoV OC43)
Human coronavirus 229E(hCoV 229E)
Enterovirus (EntV)
Human metapneumovirus (hMPV)

Group 2
Influenza virus A(Inf A)
Influenza virus B(Inf B)
Respiratory syncytial virus(RSV)
Adenovirus(AdV)
Human bocavirus(hBoV)
Rhinovirus(RV)

 

Analytical Sensitivity

10-102 copies.

Features

HotStart PCR System: Ultra highly-specific and sensitive results.
Multiplex RT-PCR: Multiple targets in a single reaction.
Reliable System: Automatic PCR control and internal control.
Easy-to-use Master Mix: Just add the template and primer.

Contents

2X Multiplex PCR Smart Mix
Primer Mixture
Standard Marker
Control Templates
Nuclease-free Water

References

1.Fouchier RAV, Munster A, Wallenstein TM, Bestebroer S, Herfst D, Smith GF, et al. J Virol. 2002;79:2814-2822

2.Lamb RA, Krug R. Field Virology, third ed. Lippincott-Raven Philadelphia. 1996;1353-1395.

3.Ha Y, Stevens, DJ, Wiley DC. The EMBO Journal. 2002;21;865-875

4.Russell RJ, Gamblin SJ, Haire LF, Stevens DJ, Xiao B, Ha Y, et al. Virology. 2004;287-296