The ApoE Genotyping Kit is designed to screen for Alzheimer’s disease using a Real-Time allele-specific Multiplex PCR with the human genomic DNA.
Apolipoprotein E (ApoE) consists of three isoforms: E2, E3 and E4. These three isoforms make six genotypes, (homozygous genotypes: E2/E2, E3/E3 E4/E4 and heterozygous genotypes: E2/E3, E2/E4, E3/E4). Isoform E2 is usually known as a protective isoform whereas isoform E4 is generally responsible for diseases. The predominant abnormalities are due to a single nucleotide polymorphism (SNP) of codon 112 (Cys/Arg) and codon 158 (Arg/Cys). The polymorphism has functional effects on the lipoprotein metabolism. ApoE is the primary ligand for two receptors, LDL receptors found in the liver and other tissues and ApoE specific receptors found in the liver. The co-ordinate interaction of these lipoprotein complexes with their receptors forms the basis for the metabolic regulation of cholesterol. ApoE has been found to be associated with elevated cholesterol levels or lipid derangements that lead to various clinical problems like coronary heart diseases, strokes, periphery artery diseases, and diabetes mellitus. In addition to the genotype-phenotype associations with the cardiovascular disease, the alleles and isoforms of ApoE have been related to dementias, most commonly Alzheimer’s disease. Determining the genotype of ApoE is thus considered to be an important test for patients with the disease, as well as a precautionary test for healthy people.
Blood, Buccal epithelial cell, Hair (root)
(E2/E2, E3/E3, E2/E4, E3/E3, E3/E4, E4/E4)
HotStart PCR System: Ultra highly-specific and sensitive results.
DnaFree™ System: No host genomic DNA contamination.
UDG System: No carryover contamination.
Multiplex PCR : Multiple targets in a single reaction.
Reliable System: Confirmation by control template.
Easy-to-use Master Mix: Just add the template and primer.
2X Multiplex PCR Smart Mix
Primer & Probe
Control Templates (E2/E2, E3/E3, E4,E4)
ABI 7500, 7500 Fast
Bio Rad CFX96™
1.Lepara O, Valjevac A, Alajbegovic A, Zaciragic A,Nakas-Icindic E (August 2009). “Decreased serum lipids in patients with probable Alzheimer’s disease”. Bosn J Basic Med Sci 9 (3):215-20
2.Golomb BA, Evans MA (2008). “Statin adverse effects : a review of the literature and evidence for a mitochondrial mechanism”. Am J Cardiovasc Drugs 8 (6):373-418
3.Hoek KS, Schlegel NC, Eichhoff OM, et al. (2008). “Novel MITF targets identified using a two-step DNA microarray strategy”. Pigment Cell Melanoma Res. 21 (6):665-76
4.Singh PP, Singh M, Mastana SS (2006). “ApoE distribution in world populations with new data from India and the UK.”. Ann.Hum. Biol. 33 (3)279-308