DiaPlexQ™ Dengue Virus Detection Kit

The DiaPlexQ™Dengue Fever Detection Kit is designed to detect 4 serotypes of the Dengue virus using the Multiplex PCR technology which can detect multiple specific target genes in a single PCR.

Pathogen Information

Dengue fever and Dengue hemorrhagic fever are caused by a RNA flavi virus that is spread by the bites of mosquitoes, most commonly Aedes aegypti, which is found in tropic and subtropic region. As many as 100 million people are infected yearly. There are four known serotypes of the flavivirus that cause dengue fever, DEN-1, DEN-2, DEN-3 and DEN-4. Infection of one dengue fever provides lifelong immunity from that serotype, but not from the other serotypes. As there are not yet any vaccine to prevent to infection with Dengue fever virus. It is essential to identify the strain of the infecting virus, immune status of the patient as early as possible.

Detection Technology

Real-Time OneStep RT-PCR

Specimens

Blood

Target Organisms

Dengue Fever Virus serotype 1 (DEN-1)
Dengue Fever Virus serotype 2 (DEN-2)
Dengue Fever Virus serotype 3 (DEN-3)
Dengue Fever Virus serotype 4 (DEN-4)

Analytical Sensitivity

10-102 copies

Features

HotStart PCR System: Ultra highly-specific and sensitive results.
Multiplex PCR: Multiple targets in a single reaction.
OneStep RT PCR: Easy and efficient process.
Reliable System: Confirmation by control template.
Easy-to-use Master Mix: Just add the template and primer.
CE Certification.

Contents

OneStep qRT-PCR Enzyme Mix
10X OneStep qRT-PCR Buffer
Primer & Probe Mixture
Control Template
RNase-free Water

Compatible Real-Time Thermal Cyclers

ABI 7500, 7500 Fast
Bio Rad CFX96™

References

1. WHO(2009) Dengue Guidelines for Diagnosis, Treatment, Prevention and Control. Geneva: World Health Organization.

2. Global Strategy For Dengue Prevention And Control. World Health Organization. 2012.pp.1617

3. Peeling, R.W.H Artsob, J.L. Pelegrino, P. Buchy, M.J.Cardosa, S.Devi, D.A Enria, J. Farrar, D.J. Gubler, M.G.Guzman, S.B. Halstead,E. Humsperger,S.Kliks, H.S.Margolis, C.M.Nathanson, V.C. Nguyen, N. Rizzo, S.Vazquez, and S. Yoksan.2010. Evaluation of diagnostic test: Dengue. Nat Rev Microbiol 8:530-38