The DiaPlexQ™ Influenza Virus A Subtype Detection Kit is designed to detect 6 types of Influenza Virus A Subtype pathogens using multiplex RT-PCR technology which can detect multiple specific target genes in a single PCR.
Influenza virus A (H1N1) is a subtype of Influenza virus A and the most common cause of influenza (flu) in humans. Some strains of H1N1 are endemic in humans and cause a small portion of all influenza-like illness and a large portion of all seasonal influenza. H3N2 is currently endemic in both human and pig populations. It evolved from H2N2 through an antigenic shift and caused the Hong Kong Flu pandemic of 1968 and 1969 that killed almost 750,000 people. The dominant strain of annual flu in January 2006 was H3N2. Since November 2003, nearly 400 cases of human infection with the highly pathogenic Avian Influenza A (H5N1) viruses have been reported by more than a dozen countries in Asia, Africa, the Pacific, Europe and the Near East. Highly pathogenic Avian Influenza A (H5N1) virus infections occur in both poultry and humans. Furthermore, although H7N2, H7N3, H7N7 and H9N2 are classified as relatively low pathogenic infections, they have been reported in humans in the past few years and they can potentially develop into pandemic infections.
Nasopharyngeal swab, Nasopharyngeal aspirate.
Bronchoalveolar lavage (BAL).
HotStart PCR System: Ultra highly-specific and sensitive results.
Multiplex RT-PCR: Multiple targets in a single reaction.
Reliable System: Internal control.
Easy-to-use Master Mix: Just add the template and primer.
OneStep qRT-PCR Enzyme Mix.
5X OneStep qRT-PCR Buffer.
Primer and Probe Mixture.
Compatible Real-Time Thermal Cyclers
ABI 7500, 7500 Fast
Bio Rad CFX96™
1.Lin YP, Shaw M, Gregory V, Cameron K, Lim W, Klimov A, et al. Avian-to-human transmission of H9N2 subtype influenza A viruses: relationship between H9N2 and H5N1 human isolates. Proc Natl Acad Sci USA. 2000; 97:9654-58
2.CDC, Interim guidance on specimen collection, processing, and testing for patients with suspected novel influenza A(H1N1) virus infection, May 13, 2009