DiaPlexQ™ Mycoplasma 9 Detection Kit

The Mycoplasma 9 Detection Kit is designed to detect 9 types of the Mycoplasma species in cell culture contaminants using the Multiplex PCR technology which can detect multiple specific target genes in a single PCR.

Pathogen Information

Mycoplasma infections have been relatively common in cell culture: (5-35% of all cell cultures are infected and much higher in some countries that do not practice systematic detection and elimination). Biomedical product Mycoplasma infections which are typically difficult to detect during routine cell culture work can cause physiological and morphological distortions that affect experimental results. Furthermore, a Mycoplasma infection in one cell culture has the potential to spread to yet uninfected laboratory cell cultures.

Detection Technology

Real-Time PCR.

Specimens

Culture media, Suspension media, Raw material and Final material of Biopharmaceuticals.

Target Organisms

Acholeplasma laidlawii
Mycoplasma synoviae
Mycoplasma fermentans
Mycoplasma hyorhinis
Mycoplasma gallisepticum
Mycoplasma orale
Mycoplasma arginini
Mycoplasma pnuemoniae
Spiroplasma citri

Analytical Sensitivity

10-102 copies.

Features

HotStart PCR System: Ultra highly-specific and sensitive results.
UDG System: No carryover contamination.
Multiplex PCR: Multiple targets in a single reaction.
Reliable System: Internal control.

Contents

2X Multiplex Real-Time PCR Smart mix (with UDG)
Primer and Probe mixture.
Control templates.
Nuclease-free Water.

Compatible Real-Time Thermal Cyclers

ABI 7500 ,7500 Fast
Bio Rad CFX96™

References

1.McGarrity G.J., Steiner, T. and Vanaman, V.Detection
of Mycoplasmal Infection of Cell Cultures by DNA Flu
orochro me Staining. In Methods in Mycoplasmology,
Vol/ 2, J.G. Tylly and S.Razin, eds. (Academic Press,
New York, 1983) pp. 183-190.

2.McGarrity G.J., Vanaman, V. and Sarama, J. Cyto
genetic Effects of Mycoplasmal Infection of Cell Cult
ures: A Review. In Vitro 20 (1):1-18. (1984)

3.Doyle A, Gri ffi ths JB., 1998. The cell: selection and
standardization. In:Cell and tissue culture: laborato
ry procedures in biotechnology. Doyle, A and Griffiths
JB (Eds), Wiley and Sons, Ltd pp.35-52

4.Asarnow, D. et al. Validation and international reg
ulatory experience for a mycoplasma touchdown
PCR assay. Biol ogicals 38:224-231 (2010)