DiaPlexQ™ Mycoplasma Detection Kit

The DiaPlexQ™ Mycoplasma Detection Kit is designed to detect 65 types of the Mycoplasma species (54 Mycoplasma sp., 3 Acholeplasma, 6 Spiroplasma sp., 2 Ureaplasma., etc) in cell culture contaminants using the Multiplex PCR technology which can detect multiple specific target genes in a single PCR.

 

Pathogen Information
Mycoplasma infections have been relatively common in cell culture: (5-35% of all cell cultures are infected and much higher in some countries that do not practice systematic detection and elimination). Biomedical product Mycoplasma infections which are typically difficult to detect during routine cell culture work can cause physiological and morphological distortions that affect experimental results. Furthermore, a Mycoplasma infection in one cell culture has the potential to spread to yet uninfected laboratory cell cultures.
Detection Technology
Real-Time PCR.
Specimens
Culture media, Suspension media, Raw material and Final material of Biopharmaceuticals
Target Organisms
Mycoplasma of 65 species:
(54 Mycoplasma sp., 3 Acholeplasma sp.
6 Spiroplasma sp., 2 Ureaplasma sp., etc)
Analytical Sensitivity
10-102 copies.
Features
HotStart PCR System: Ultra highly-specific and sensitive results.
DnaFree™ System: No host genomic DNA contamination.
UDG System: No carryover contamination.
Multiplex PCR: Multiple targets in a single reaction.
Reliable System: Automatic PCR control.
Easy-to-use Master Mix: Just adding template and primer.
Contents
2X Multiplex Real-Time PCR Smart mix (with UDG)
Primer and Probe mixture.
Control templates.
Nuclease-free Water.
Compatible Real-Time Thermal Cyclers
ABI 7500 ,7500 Fast
Bio Rad CFX96™
References
1.McGarrity G.J., Steiner, T. and Vanaman, V.Detection of Mycoplasmal Infection of Cell Cultures by DNA Fluorochro me Staining. In Methods in Mycoplasmology, Vol/ 2, J.G. Tylly and S.Razin, eds. (Academic Press, New York, 1983) pp. 183-190.

2.McGarrity G.J., Vanaman, V. and Sarama, J. Cyto genetic Effects of Mycoplasmal Infection of Cell Cultures: A Review. In Vitro 20 (1):1-18. (1984)

3.Doyle A, Gri ffi ths JB., 1998. The cell: selection and standardization. In:Cell and tissue culture: laboratory procedures in biotechnology. Doyle, A and Griffiths JB (Eds), Wiley and Sons, Ltd pp.35-52

4.Asarnow, D. et al. Validation and international regulatory experience for a mycoplasma touchdown PCR assay. Biol ogicals 38:224-231 (2010)