DiaPlexQ™ PneumoPatho 13 Detection Kit

The DiaPlexQ™ PneumoPatho 13 Detection Kit is designed to detect 13 species of Pneumonia using the Multiplex PCR technology which can detect multiple specific target genes in a single PCR.

Pathogen Information

Pneumonia is usually caused by infectious organisms, mostly by viruses or bacteria. Pneumonia affects approximately 450 million people globally per year, seven percent of the global population, and results in about 4 million deaths per year, mostly in third world countries.

Detection Technology

Real-Time PCR

Specimens

Nasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL) Nasal swab, Oropharyngeal swab, Sputum.

Target Organisms

Group 1: M. pneumoniae, K. pneumoniae, C. pneumonia
Group 2: S. pneumoniae, S. aureus, L. pneumophila
Group 3: P. aeruginosa, M. catarrhalis, B. pertussis
Group 4: H. influenza, A. baumannii, M. tuberculosis/avium

Analytical Sensitivity

10-102 copies

Features

HotStart PCR System: Ultra highly-specific and sensitive results.
Multiplex PCR: Multiple targets in a single reaction.
Easy-to-use Master Mix: Just add template and primer.

Contents

2X Multiplex Real-Time PCR Smart Mix (with UDG) (PneumoPatho 13)
Primer and Probe Mixture (I-IV)
Control Template (I-IV)
Nuclease-free Water

Compatible Real-Time Thermal Cyclers

ABI 7500, 7500 Fast

Bio Rad CFX96™

References

1. Micheal J McConnell et al. Quantitative Real-Time PCR for Detection of Acinetobacter baumannii Colonization in the Hospital Environment. Journal of Clinical microbiology p. 1412-1414.

2. Ko WC, Paterson DL, Sagnimeni AJ, et al. Community-acquired Klebsiella pneumonia bacteremia: global differences in clinical patterns. Emerg Infect Dis 2002; 8:160-6.

3. Gierczynski R, Jagielski M, Rastswicki W, Klauzewski S (2007) Multiplex-PCR assay for identification of Klebsiella pneumonia isolates carrying the chain loci for K1 and K2 capsule biosynthesis. Pol J Microbiol 56: 153-156.

4. Brittain-Long, R., Nord, S., Olofsson, S., Westin, J., Anderson, L.M. & Lindh, M. (2008). Multiplex real-time PCR for detection of respiratory tract infections. Journal ofClinical Virology 41(1): 53-56.

5. 1.Chan, Y.R. & Morris, A. (2007). Molecular diagnostic methods in pneumonia. Current Opinion in Infectious Diseases 20(2): 157-164.

6. 1.Daxboeck, F., Khanakah, G., Bauer, C., Stadler, M., Hofmann, H. & Stanek, G. (2005). Detection of Mycoplasma pneumoniae in serum specimens from patients with mycoplasma pneumonia by PCR. International Journal of Medical.

7. 1.Johansson, N., Kalin, M., Tiveljung-Lindell, A., Giske, C.G. & Hedlund, J. (2010). Etiology of community-acquired pneumonia: increased microbiological yield with new diagnostic methods. Clinical Infectious Diseases 50(2): 202-209.