DiaPlexQ™ PneumoPatho 6 Detection Kit

The DiaPlexQ™ PneumoPatho 6 Detection Kit is designed to detect 6 major species of Pneumonia using the Multiplex PCR technology which can detect multiple specific target genes in a single PCR.

Pathogen Information

 

Pneumonia is usually caused by infectious organisms mostly by viruses or bacteria. Pneumonia affects approximately 450 million people globally per year, seven percent of the global population, and results in about 4 million deaths per year, mostly in third world countries.

Detection Technology

Real-Time PCR

Specimens

Nasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL) Nasal swab, Oropharyngeal swab, Sputum.

 

Target Organisms

Group 1: M. pneumoniae, K. pneumoniae, C. pneumonia
Group 2: S. pneumoniae, S. aureus, L. pneumophila
Analytical Sensitivity
10-102 copies.

Features

HotStart PCR System: Ultra highly-specific and sensitive results.
Multiplex PCR: Multiple targets in a single reaction.
Easy-to-use Master Mix: Just add template and primer.

Contents

RT-PCR Enzyme Mix
Primer and Probe Mixture
Control Template
Nuclease-free Water

Compatible Real-Time Thermal Cyclers

ABI 7500

Bio Rad CFX96™

References

1. Micheal J McConnell et al. Quantitative Real-Time PCR for Detection of Acinetobacter baumannii Colonization in the Hospital Environment. Journal of Clinical microbiology p. 1412-1414.

2. Ko WC, Paterson DL, Sagnimeni AJ, et al. Community-acquired Klebsiella pneumonia bacteremia: global differences in clinical patterns. Emerg Infect Dis 2002; 8:160-6.

3. Gierczynski R, Jagielski M, Rastswicki W, Klauzewski S (2007) Multiplex-PCR assay for identification of Klebsiella pneumonia isolates carrying the 첸 loci for K1 and K2 capsule biosynthesis. Pol J Microbiol 56: 153-156.