DiaPlexQ™ RV16 Detection Kit

The DiaPlexQ™ RV16 Detection Kit is designed to detect 19 major respiratory viruses using the Multiplex RT-PCR technology which can detect multiple specific target genes in a single PCR.

Pathogen Information

Viral infections are among the leading causes of respiratory disease in children. Most of these infections are caused by respiratory syncytial virus (RSV), influenza virus A or B (InfA or InfB), parainfluenza virus (PIV), rhinovirus (RV) or adenovirus (AdV). Several recently discovered viruses, such as human metapneumovirus (hMBV), human bocavirus (hBoV), Enterovirus (EntV) and the human coronaviruses (hCoVs) 229E and OC43, have been identified as potential respiratory pathogens. The DiaPlexQ™ RV16 Detection Kit is also able to identify Middle Eastern Respiratory Syndrome (MERS).

Detection Technology

Real-Time OneStep RT-PCR
Specimens
Nasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL), Nasal swab, Oropharyngeal swab, Sputum.

Target Organisms

Group 1: Parainfluenza virus I (PIV-1)
Parainfluenza virus II (PIV-3)
Parainfluenza virus III (PIV-3)
Parainfluenza virus IV (PIV-4)
Group 2: Influenza virus A (Inf A)
Influenza virus B (Inf B)
Respiratory Syncytial virus A and B (RSV A/B)
Group 3:Adenovirus (AdV)
Group 4:Bocavirus (BoV)
Enterovirus (EntV)
Metapneumovirus (MPV)
Group 5:Rhinovirus (hRV A/B/C)
Alpha Coronavirus 229E, NL63 (CoV 229E, CoV NL63)
Beta Coronavirus OC43, MERS (CoV OC43/CoV MERS)

Analytical Sensitivity

102 Copies

 

Features

HotStart PCR System: Ultra highly-specific and sensitive results.
Multiplex PCR: Multiple targets in a single reaction.
Easy-to-use Master Mix: Just add the template and primer.

Contents

OneStep RT-PCR Enzyme Mix
2X OneStep RT-PCR Buffer
Primer and Probe Mixture (I-V)
Control Template (I-V)
RNase-free Water
Compatible Real-Time Thermal Cyclers
ABI 7300, 7500, 7500Fast

Bio Rad CFX96™

References

1. Fouchier RAV, Munster A, Wallenstein TM, Bestebroer S, Herfst D, Smith GF, et al. J Virol. 2002;79:2814-2822

2. Lamb RA, Krug R. Field Virology, third ed. Lippincott-Raven Philadelphia. 1996; 1353-1395.

3. Ha Y, Stevens, DJ, Wiley DC. The EMBO Journal. 2002;21;865-875.

4. Russell RJ, Gamblin SJ, Haire LF, Stevens DJ, Xiao B, Ha Y, et al. Virology. 2004;287-296.