HotStart PCR system (antibody-mediated PCR enzyme)
Long-range PCR (<40kb)
Low error rate
High productivity and sensitivity
DiaStar™ EF-Taq DNA Polymerase is an ideal enzyme for long-range PCR amplification maintiaining the high specificity and high sensitivity characteristics of antibody-mediated HotStart polymerase. It activates automatically at 95˚C inhibiting non-specific DNA amplification. Also, its high productivity characteristic is suitable for difficult PCR amplification due to high GC content or complex template structure, or for low concentrated template DNA amplification. DiaStar™ EF-Taq DNA Polymerase enables amplification of long-range PCR of up to 40kb like other long-range PCR polymerase. Also, to overcome error in the case of long-range PCR, DiaStar™ EF-Taq DNA Polymerase consists of two types of DNA polymerase from Pyrococcus furiosus & Thermus aquaticus for proof-reading function. It has a lower error rate than other long-range PCR enzymes or HotStart enzymes.
Source: Pyrococcus furiosus & Thermus aquaticus.
5‘ → 3’ exonuclease activity: Yes.
3‘ → 5’ exonuclease activity: Yes (week).
Amplification size: < 40 kb.
HotStart activity (antibody-mediated HotStart): Yes.
High productivity PCR.
Diastar™ EF–Taq DNA Polymerase (2.5 U/㎕)
10X EF-Taq Reaction Buffer (25 mM MgCl2 included)
5X Band Doctor™
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP)
Stable for 2 years and 3 months in the case of storage at -20, or
7 days at 25℃ before activity begins to reduce.