Quick ligation enzyme
Ligation time : 5 min
Connection of cohesive end / blunt end PCR product
DiaStar™ Quick DNA Ligase is a ligation enzyme and it is designed to connect and combine 5′-phosphate and 3-‘OH phosphodiester by using of ATP as a cofactor which is laid in a parallel. This product is useful to connect both of cohesive end and blunt end PCR product and needs to be react for 5 minutes at room temperature for ligation. Also, DiaStar™ Quick DNA Ligase is used to connect nick which is formed in a single strand.
Source: An E. coli strain with a recombinant T4 DNA Ligase gene from Bacteriophage
Joining of double-stranded DNA with cohesive or blunt end.
Joining of linker or adaptors to blunt-ended DNA.
Repairing nicks in duplex DNA, RNA or DNA-RNA hybrids.
DiaStar™ Quick DNA Ligase.
2X Ligase Buffer.
Storage & Expiration Date
Stable for 2 years and 3 months in the case of storage at -20, or
7 days at 25℃ before activity begins to reduce.