HotStart PCR system for stringent specificity (antibody-mediated PCR enzyme)
Amplification of very low copy DNA
High productivity and high sensitivity
DiaStar™ Taq DNA Polymerase is a recombinant Taq DNA polymerase complex with a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 95˚C, thereby providing an automatic HotStart for Taq DNA polymerase. HotStart PCR provides increased sensitivity, specificity and productivity, while allowing assembly of reactions at room temperature. The use of this antibody helps reduce PCR optimization requirements, reaction set-up time and effort, handling of reaction components, and contamination risk. DiaStar™ Taq DNA Polymerase is suited to the amplification of a low copy target DNA or difficult PCR due to high GC content and complex template structure. It is also effective at inhibiting the formation of non-specific PCR product or primer-dimer.
Source: Thermus aquaticus.
5’→3’ exonuclease activity: Yes.
3’→5’ exonuclease activity: No.
Amplification size: ＜ 5 kb.
Amplification of low copy DNA
DiaStar™ Taq DNA Polymerase (5 U/㎕)
10X Taq Reaction Buffer (25 mM MgCl2 included)
5X Band Doctor™
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP)
Stable for 2 years and 3 months in the case of storage at -20, or
7 days at 25℃ before activity begins to reduce.