AMP™ EF-Taq DNA Polymerase

Long-range PCR (<40kb)
Low error rate
Dual advantage of DNA polymerase from Pyrococcus furiosus and Thermus aquaticus

Description

AMP™ EF-Taq DNA Polymerase is ideal for long-ranged PCR of up to 40 kb. This enzyme is optimized with a proper blend ratio of two enzymes, Pyrococcus furiosus and Thermus aquaticus, making dual advantages. It has a lower error rate (approximately four times less) compared to the AMP™ Taq DNA Polymerase due to its 3’ to 5’ exonuclease activity for proof-reading, and also enables the user to get a large amount of PCR product due to its greater processivity than that of other Taq polymerase. AMP™ EF-Taq DNA Polymerase generates sufficient product with an A overhang, (eliminating the need to add A overhangs post PCR) to apply TA cloning of PCR product directly. AMP™ EF-Taq DNA Polymerase is useful for amplification which is required for higher sensitivity and productivity in long-range PCR. The optional 5x Band Doctor™ helps amplification of difficult PCR caused by high GC content or complex template structure.

Features

Source: Pyrococcus furiosus & Thermus aquaticus.
5‘ → 3’ exonuclease activity: Yes.
3‘ → 5’ exonuclease activity: No.
Amplification size: < 5 kb.
A-tailing: Yes.
Error rate: 12-13 bp error/ 106 bp.

Application

Routine PCR
PCR screening
TA cloning
Colony PCR

Contents

AMP™ EF-Taq DNA Polymerase (2.5 U/㎕)
10X EF-Taq Reaction Buffer (25 mM MgCl2 included)
5X Band Doctor™
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP)

Storage & Expiration Date

-20℃
Stable for 2 years and 3 months in the case of storage at -20, or
7 days at 25℃ before activity begins to reduce.

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