HPV 3.5

LCD-Array Kit

  • Fast
    120 min PCR / 45 min array protocol / 5 min analysis Fast First results after ~3 hours (excl. sample preparation)

 

  • Sensitive
    Two primer mixes generating amplicons of ~430 bp and ~150 bp, suited
    for formalin-fixed, paraffin-embedded (FFPE) tissue sections

 

  • Specific
    32 specific capture probes immobilized on polymer chips detecting
    32 HPV types without cross reactivity

 

  • Robust
    Non-fluorescent detection for easy read-out and long term storage;
    fully automated read-out by SlideReader software, minimal instrumentation

 

 

Product Information

Assay Principle

Biotinylated PCR products are generated from sample material containing extracted HPV DNA. The labeled amplicons are hybridized to subtype specific capture probes immobilized on the LCD-Chip surface. Following a short wash routine, comprising high stringency, visualization of bound amplicons is mediated by an enzyme-substrate cascade. Each LCD-Chip contains eight identical micro arrays separated in small reaction chambers. Each array can be addressed individually, allowing the parallel analysis of eight samples on one chip.

 

Specificity & Sensitivity

The binding sites for the supplied PCR* primer mixes are well conserved among the recognized HPV types and the probes spotted on the array have been thoroughly evaluated for their type specificity. Two primer sets for PCR amplification are provided. One based on the published and commonly used My11/My09 system and a second, producing shorter amplicons of ~150 bp in length. Due to the shorter fragment length, the primer mix HPV 125 might be the better choice for starting material where degradation could not be excluded. Combining both PCR reactions, the assay is very well suited for the analysis of HPV typ


Detected HPV types

hpv2

Fig.1:

fig1
Parallel analysis of eight samples on one LCD-Chip. The array field spacing is compatible with standard multi pipettes.

Fig.2 :

Capture d’écran 2015-01-21 à 17.36.08
Detection of HPV types from formalin-fixed, paraffin-embedded (FFPE) tissue sections. A: single type infection (HPV Type 16)
B: multiple type infection (HPV Type 06, 35, 66) A B
* The Polymerase Chain Reaction (PCR) process is covered by U.S. patents owned by Hoffmann-La Roche, Inc. and patents owned by F. Hoffmann-La Roche Ltd. No license under these patents to use the PCR process is conveyed expressly or by implication to the purchaser by the purchase of this product.

 

Software

hpv3

SlideReader Vers. 7.01

• Fully automated image analysis
• comprehensive data reports in HTML format
• fast & intuitive navigation
• multiple user accounts

 

Instrumentation

hpv4

CHIP-Scanner PF2700

• Transmission light scanner
for LCD-Arrays,
• 10 µm resolution
• 16 bit grey scale TIFF images

CHIP Spin FVL2400

• Bench Top mini centrifuge
• 2400 rpm, constant
• Adaptor for LCD-Arrays
• incl. 3 rotors

hpv5

hpv1