AMP™ Pfu DNA Polymerase

High fidelity
Produces blunt ended PCR

Description

AMP™ Pfu DNA Polymerase, a product of recombinant type DNA polymerase from Pyrococcus furiosus, is a suitable product for high-fidelity PCR amplification. The error rate of the AMP™ Pfu DNA Polymerase is 12 times lower than that of the AMP™ Taq DNA Polymerase due to its intrinsic 3’ to 5’ exonuclease proof-reading activity. It produces blunt ended PCR products and enables blunt cloning directly using a AMP  T-Blunt™ PCR Cloning Kit without A-tailing step. Optional 5x Band Doctor™ helps amplification of difficult PCR caused by High GC content or complex template structure.

Features

Source: Pyrococcus furiosus.
5‘ → 3’ exonuclease activity: No.
3‘ → 5’ exonuclease activity: Yes.
Amplification size: < 3 kb.
Hot start activity (Chemical-mediated Hot start): No.
A-tailing: No.
Error rate: 1-2 bp error / 106 bp.

Application

High fidelity PCR.
Site-directed mutagenesis.
Blunt end form cloning.

Contents

AMP™ Pfu DNA Polymerase (2.5 U/㎕)
10X Pfu Reaction buffer (25mM MgCl2 included)
5X Band Doctor™
Each 10mM dNTP Mix (dATP, dTTP, dGTP, dCTP)

Storage & Expiration Date

-20℃
Stable for 2 years and 3 months in the case of storage at -20, or
7 days at 25℃ before activity begins to reduce.

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