AMP™ Pfu-X DNA Polymerase

Ultra-high fidelity and processivity.
Long-ranged PCR up to 20 kb.
Produces blunt ended PCR.

Description

The AMP™ Pfu-X DNA Polymerase is an ultra-high fidelity Pfu DNA polymerase for advanced proofreading with superior processivity. The enzyme from Pyrocuccus furiosus is a modified DNA polymerase with enhanced proofreading activity and high template binding affinity. The enhanced proofreading activity (three times higher than AMP™ Pfu DNA polymerase) dramatically improves processivity and accurate PCR products. Additionally, the increased processivity (four times faster than AMP™ Pfu DNA polymerase) allows up to 75% reduction in PCR protocol times for faster results.

AMP™ Pfu-X DNA Polymerase is supplied with an optimized buffer and 5X Band Doctor™ for successful PCR amplification in High GC content and other difficult PCR targets. It produces blunt ended PCR products and enables blunt cloning directly using SolGen T-Blunt™ PCR Cloning Kit without A-tailing step.

Features

Source: Pyrococcus furiosus.
5‘ → 3’ exonuclease activity: No.
3‘ → 5’ exonuclease activity: Yes.
Amplification size: < 20 kb.
Hot start activity (Chemical-mediated Hot start): No.
A-tailing: No.
Error rate: 1-2 bp error / 106 bp.

Application

High fidelity PCR.
Long-range PCR.
Site-directed mutagenesis.
Blunt end form cloning.

Contents

AMP™ Pfu-X DNA Polymerase (2.5 U/㎕)
10X Pfu-X Reaction buffer (25mM MgCl2 included)
5X Band Doctor™
Each 10mM dNTP Mix (dATP, dTTP, dGTP, dCTP)

Storage & Expiration Date

-20℃
Stable for 2 years and 3 months in the case of storage at -20, or
7 days at 25℃ before activity begins to reduce.

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