Ultra-high fidelity and processivity.
Long-ranged PCR up to 20 kb.
Produces blunt ended PCR.
The AMP™ Pfu-X DNA Polymerase is an ultra-high fidelity Pfu DNA polymerase for advanced proofreading with superior processivity. The enzyme from Pyrocuccus furiosus is a modified DNA polymerase with enhanced proofreading activity and high template binding affinity. The enhanced proofreading activity (three times higher than AMP™ Pfu DNA polymerase) dramatically improves processivity and accurate PCR products. Additionally, the increased processivity (four times faster than AMP™ Pfu DNA polymerase) allows up to 75% reduction in PCR protocol times for faster results.
AMP™ Pfu-X DNA Polymerase is supplied with an optimized buffer and 5X Band Doctor™ for successful PCR amplification in High GC content and other difficult PCR targets. It produces blunt ended PCR products and enables blunt cloning directly using SolGen T-Blunt™ PCR Cloning Kit without A-tailing step.
Source: Pyrococcus furiosus.
5‘ → 3’ exonuclease activity: No.
3‘ → 5’ exonuclease activity: Yes.
Amplification size: < 20 kb.
Hot start activity (Chemical-mediated Hot start): No.
Error rate: 1-2 bp error / 106 bp.
High fidelity PCR.
Blunt end form cloning.
AMP™ Pfu-X DNA Polymerase (2.5 U/㎕)
10X Pfu-X Reaction buffer (25mM MgCl2 included)
5X Band Doctor™
Each 10mM dNTP Mix (dATP, dTTP, dGTP, dCTP)
Stable for 2 years and 3 months in the case of storage at -20, or
7 days at 25℃ before activity begins to reduce.