AMP™ Real-Time PCR Series – Detection with EvaGreen™

Rapid & accurate real-time quantification of target DNA
High specific detection using h-Taq DNA polymerase (HotStart)
Accurate detection of a wide range of template amounts

Description

AMP™ Real-time PCR Series is providing optimizing system for easy-use using intercalating reagent in real-time PCR amplification. Included AMP™ h-Taq DNA Polymerase helps accurate and reliable result to enable target-specific amplification due to its HotStart characteristic by inhibition of primer- dimer or non-specific binding. Optional or mixed fluorescent dye, EvaGreen™ is available to use as an intercalating reagent, even though high resolution melting (HRM) analysis is applicable for genetic analysis such as SNP genotyping, mutation discovery or CpG methylation.

Application

DNA using real-time PCR.
Real-time quantification of RNA target.
High Resolution Melting (HRM) analysis.

Features of the EvaGreen™ Dye

Spectral property: λabs / λem = 500 / 530 nm (DNA bound), λabs = 471 nm (w/o DNA).
No PCR inhibition by dye concentration.
Very bright and stable fluorescence.
Equally binding to G+C rich and A+T rich regions.
Suitable for HRM analysis.
Non-mutagenic, non-cytotoxic.
Storage: -20℃ (where the light is blocked and no moisture).

Contents

EvaGreen™ dye (20X in water).
AMP™ h-Taq DNA Polymerase (2.5 U/㎕).
10X Reaction Buffer (25 mM MgCl2 included).
5X Band Doctor™.
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP).

Storage & Expiration Date

-20℃
Stable for 6 months in the case of storage at -20, or
7 days at 25℃ before activity begins to reduce.

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