Isothermal amplification using Phi29 DNA polymerase
Rolling circle amplification (RCA) Kit
Direct amplification from various sample
(colony, liquid culture, glycerol stock or purified sample)
Direct sequencing after amplification without further purification step
SEQ-TempliGen™ RCA Kit is designed to amplify circular DNA from various sample(liquid culture, glycerol stock or purified sample) based on isothermal amplification using Phi29 DNA polymerase, and even it is very efficient to amplify of low copy plasmid, BAC, Fosmid and repeated structure of plasmid.
The amplification step is not required of expensive machine and extra time for cell culture, and the amplified DNA is used sequencing reaction directly without further purification step. SEQ-TempliGenTM RCA Kit enables producing micrograms of DNA from pictograms of input circular
DNA with the provided buffer.
Source: Bacillus subtilis phage Phi29.
Activation temperature: 30.
3‘ → 5’ exonuclease activity: Yes.
Amplification size: > 70 kb.
Amplification fold: 103 – 109.
Error rate: 1 bp error / 106 – 107 bp.
RCA for sequencing.
Low copy plasmid amplification.
RCA Denaturation Buffer (DB).
RCA Reaction Buffer (RB).
RCA BSA Buffer (BSA).
RCA Enzyme mixture (EM).
Stable for 1 year in the case of storage at -20, or
7 days at 25℃ before activity begins to reduce.