Removing Uracil-containing DNA
Elimination of carry-over contamination
Applied to PCR based diagnostics for highly accurate result
DAQ™ Uracil-DNA Glycosylase excises Uracil from dU-containing DNA by cleaving the N-glycosidic bond between the uracil base and the sugar backbone. This cleavage generates alkali sensitive apyrimidinic sites that are blocked from replication by DNA polymerase or prevented from becoming a hybridization site. Double and single-stranded dU-containing DNA are substrates for Uracil-DNA Glycosylase whereas RNA and normal dT-containing DNA are not.
DAQ™ Uracil-DNA Glycosylase is useful to reduce the carry-over contamination in PCR based diagnostics.
Source: E. coli (K-12 strain).
Removing uracil-containing DNA.
Elimination of carry-over contamination.
Diagnosis system using PCR.
E. coli Uracil-DNA Glycosylase (1 U/㎕).
Storage Temperature & Expiration Date
Stable for 2 years and 3 months in the case of storage at -20, or
7 days at 25℃ before activity begins to reduce.