Connection of cohesive end / blunt end PCR product
AMP™ T4 DNA Ligase is a ligation enzyme and it is designed to connect and combine 5′-phosphate and 3-‘OH phosphodiester by using of ATP as a cofactor which is laid in a parallel. This product is useful to connect both of cohesive end and blunt end PCR product. AMP™ T4 DNA Ligase is used to connect nick which is formed in a single strand.
Source: Bacteriophage T4.
Joining of double-stranded DNA with cohesive or blunt end.
Joining of linker or adaptors to blunt-ended DNA.
Repairing nicks in duplex DNA, RNA or DNA-RNA hybrids.
AMP™ T4 DNA Ligase (400 U/㎕).
10X Ligase Buffer.
Stable for 2 years and 3 months in the case of storage at -20, or
7 days at 25℃ before activity begins to reduce.